Table 1 Primer sequences used in qRT-PCR
Target gene | Accession No. | Sense Primer | Antisense Primer | Tm (°C) |
|---|---|---|---|---|
OCT4 | NM002701.4 | TGGCTCTGCTGACACATC | TGGTTCGCTTTCTCTTTCG | 53.4 |
SOX2 | NM003106.2 | GAGAGAAAGAAAGGGAGAGAAG | GAGAGAGGCAAACTGGAATC | 53.0 |
NANOG | NM024865.2 | CTGGCTGAATCCTTCCTCTCC | TGATTAGGCTCCAACCATACTCC | 57.0 |
Î’-Actin | NM001101 | GGACCTGACTGACTACCTC | GCCATCTCTTGCTCGAAG | 53.9 |