Figure 4From: Targeted genome engineering in human induced pluripotent stem cells by penetrating TALENs Western blot analysis of lysate from the treated HeLa cells using an anti-FLAG antibody. HeLa cells were incubated with TALEN for 1 hour at 37°C and subsequently washed three times with 1 mg/ml heparin-PBS to remove proteins bound to the cell surface before lysis.Back to article page