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Table 4 Ligand-CAM interactions reported to support long-term hPSC culture

From: Defining synthetic surfaces for human pluripotent stem cell culture

Substrates

Ligands or ECMPs

CAM(s)

References

Tissue culture polystyrene (TCP)

Vitronectin

αVβ3/5 integrins, GAGs

[7]

Laminin-511

α6β1 integrin

[11]

Laminin E8 fragments

-

[13]

Fibronectin

α5β1 integrin

[48]

Collagen + fibronectin + laminin + vitronectin

-

[125]

Poly(L-lysine)

-

[126]

E-cadherin-IgG1Fc (StemAdhereTM)

E-cadherin

[10]

Amine-modified TCP

Cyclic-CRGDC

-

[86]

UV-treated TCP

Adsorbed serum proteins, vitronectin

-

[12]

Acrylate monomer-coated TCP

KGGNGEPRGDTYRAY

αVβ5

[9]

(Corning SynthemaxTM)

integrins - αVβ3/5

[9]

KGGPQVTRGDVFTMP

Vitronectin

integrins, GAGs

[42]

Self-assembled monolayers

GKKQRFRHRNRKG

HSPGs

[79]

LTTAPKLPKVTR

GAGs

[127]

Amino-propylmethacrylamide

BSA + non-specific proteins (adsorbed from media)

-

[14]

Hydrogels

Polyacrylamide hydrogel

GKKQRFRHRNRKG

HSPGs

[92]

PMEDSAH

Unknown. Adsorbed growth factors?

-

[99]

  1. Surfaces are arranged according to their substrate. The ligands or extracellular matrix proteins (ECMPs) that are presented from those substrates and the CAMs with which they have been shown to interact (if any) are also listed. Whole ECMPs, ECMP fragments, fusion proteins, and peptides presented by amine-modified or acrylate monomer coated TCP, protein-fouling hydrogels and polymers have all demonstrated the capacity to support hPSC culture by interacting with various integrins, E-cadherins and/or heparan sulphate proteoglycans. The surfaces listed have all been reported to support hPSC culture subject to at least the minimum cell characterisation requirements outlined in Table 1. Key references have been provided for each surface.