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Table 4 Ligand-CAM interactions reported to support long-term hPSC culture

From: Defining synthetic surfaces for human pluripotent stem cell culture

Substrates Ligands or ECMPs CAM(s) References
Tissue culture polystyrene (TCP) Vitronectin αVβ3/5 integrins, GAGs [7]
Laminin-511 α6β1 integrin [11]
Laminin E8 fragments - [13]
Fibronectin α5β1 integrin [48]
Collagen + fibronectin + laminin + vitronectin - [125]
Poly(L-lysine) - [126]
E-cadherin-IgG1Fc (StemAdhereTM) E-cadherin [10]
Amine-modified TCP Cyclic-CRGDC - [86]
UV-treated TCP Adsorbed serum proteins, vitronectin - [12]
Acrylate monomer-coated TCP KGGNGEPRGDTYRAY αVβ5 [9]
(Corning SynthemaxTM) integrins - αVβ3/5 [9]
Vitronectin integrins, GAGs [42]
Self-assembled monolayers GKKQRFRHRNRKG HSPGs [79]
Amino-propylmethacrylamide BSA + non-specific proteins (adsorbed from media) - [14]
Polyacrylamide hydrogel GKKQRFRHRNRKG HSPGs [92]
PMEDSAH Unknown. Adsorbed growth factors? - [99]
  1. Surfaces are arranged according to their substrate. The ligands or extracellular matrix proteins (ECMPs) that are presented from those substrates and the CAMs with which they have been shown to interact (if any) are also listed. Whole ECMPs, ECMP fragments, fusion proteins, and peptides presented by amine-modified or acrylate monomer coated TCP, protein-fouling hydrogels and polymers have all demonstrated the capacity to support hPSC culture by interacting with various integrins, E-cadherins and/or heparan sulphate proteoglycans. The surfaces listed have all been reported to support hPSC culture subject to at least the minimum cell characterisation requirements outlined in Table 1. Key references have been provided for each surface.