From: Generation of multi-gene knockout rabbits using the Cas9/gRNA system
Gene | gRNA/cas9 mRNA (ng/μL) | No. of injected zygotes | No. of blastocysts (%) | No. of sequenced blastocysts | No. of modified (%) | No. of biallelic-modified (%) | No. of 2 gene- modified (%) | No. of 3 gene- modified (%) | No. of 3 gene biallelic-modified (%) |
---|---|---|---|---|---|---|---|---|---|
IL2rg | Â | Â | Â | 18 | 18 (100) | 18 (100)* | Â | Â | Â |
RAG1 | 20/20/20/200 | 24 | 20 (83.3) | 19 | 19 (100) | 17 (89.5) | 18/18 (100) | 16/16 (100) | 15/16 (93.75)* |
RAG2 | Â | Â | Â | 16 | 16 (100) | 16 (100) | Â | Â | Â |