In vitro and in vivo testing of TALENs. A. Schematic depiction of the SSA test as explained in step 4 of the procedure. B. Flow cytometry analysis of HEK 293 T cells transfected with a GFP “dead” reporter shows increased GFP activity with co-transfection of a specific pair of TALEN plasmids compared to the control. C. T7 endonuclease I test result. Lanes 1 and 2 are negative controls, 3 and 5 are samples A and B before treatment, respectively, 4 and 6 are samples A and B after treatment, respectively. White arrows indicate the expected fragments after cleavage by T7 endonuclease I; bp stands for base pairs.