Differentiation of hESC to endothelial cells. (A) Diagram of the differentiation protocol. (B–D) Semi-quantitative real-time PCR analysis of gene expression in cells from days 0 to 8 of differentiation. Genes examined: (B)
BRACHYURY and ETV2. (C)
VE-CADHERIN and CD-31. (D)
KDR and CD34. (E) Flow cytometry analysis of day 7 differentiation of hESC. Percentages shown represent averages from five experiments with standard deviations. (F) Quantitative analysis of data in panel (E). Immunofluorescence of day 7 of differentiation for CD31 (G) and VE-CADHERIN (H).