Fig. 3

Characterization of subtype blood lineages from H1 or H1-GATA2 ^−/− derived HPCs. a CFU potential cells from H1 or H1-GATA2 ^−/− were restricted within CD34⁺CD43⁺ subpopulations. EC endothelial cells, MC mesenchymal cells. b Characterization of erythrocytes from H1 or H1-GATA2 ^−/−. From left to right: phase-contrast photographs of BFU and CFU-E, FACS analysis of CD235a and CD71a expression on H1 and H1-GATA2 ^−/− derived erythrocytes, and cytospin of H1 and H1-GATA2 ^−/− derived erythrocytes. c Globin analysis of erythrocytes by RT-qPCR. The results showed the mean + SEM of one single experiment with three replicates, representative of three independent experiments. d Analysis of expression of GATA1, GATA2, and GATA3 in H1 or H1-GATA2 ^−/− derived erythrocytes. The results showed the mean + SEM of one single experiment with three replicates, representative of three independent experiments. e Characterization of myeloid cells from H1 or H1-GATA2 ^−/−. Left: morphologies of indicated CFU colonies; middle: FACS analysis of indicated markers; right: cytospin photographs of indicated colonies. f FACS analysis of CD86 and CD14 expression in H1 and H1-GATA2 ^−/− derived myeloid CFU. E erythrocyte, G granulocyte, M macrophage, GM G and M, Mix G, E, and M