Fig. 4
From: Loss of Mgat5a-mediated N-glycosylation stimulates regeneration in zebrafish
mgat5a hg29 mutation enhances hair cell regeneration. a Schematic diagram of the mgat5a hg29 mutation. Locations of the two CRISPR targets are indicated by red arrows. The primers used for mutation detection and knockdown analysis are indicated by black arrows. The resulted mgat5a E3-E10 mutation deletes 49.2 kb of genomic DNA between the T1 and T2 CRISPR targets. b RT-PCR analysis of mgat5a expression in wild-type (WT) and homozygous mutant embryos. E3F/E10R primer pair produces an amplicon of 985 bp in the WT embryos and an amplicon of 244 bp in the mutant embryos. Beta-actin is used as internal reference. c Enhanced hair cell regeneration in mgat5a hg29 mutants. The graph is obtained from analyzing and afterwards genotyping 32 embryos that were generated from a pairwise incross of heterozygotes. Error bars show the s.e.m.. The difference between wild-type (WT) and homozygotes in d is significant (p = 0.02)