Skip to main content

Table 1 Culture media for human GI organoids

From: Generation of 3D human gastrointestinal organoids: principle and applications

Organ Source of organoids Basal medium EGF Noggin R-spondin1 Wnt3a Gastrin FGF10 Nicotinamide A-83-01 SB202190 PGE2 Reference
Stomach Corpus +a 50 ng/ml 10% CM 10% CM 50% CM 1 nM 200 ng/ml Facultative 2 μM (Bartfeld et al., 2015)
Corpus and antrum + 20 ng/ml 150 ng/ml 25% CM 50% CM 10 nM 150 ng/ml 10 mM 1 μM 2 μM (Schlaermann et al., 2016)
Fundus + 50 ng/ml 100 ng/ml 20% CM 50% CM 1 nM 200 ng/ml 10 mM (Bertaux-Skeirik et al., 2015)
Intestine Small intestine and colon + 50 ng/ml 100 ng/ml 1 μg/ml 100 ng/ml 10 nM 10 mM 500 nM 10 μM (Sato et al., 2011)
Small intestine and colon + -b 100 ng/ml 1 μg/ml 50% CM 10 nM -b 500 nM -b (Fujii et al., 2018)
Colon + 20 ng/ml 100 ng/ml 1 μg/ml 50% CM 1 μg/ml 10 mM 500 nMc 10 μM 10 nM (Jung et al., 2011)
Colon +a 50 ng/ml 100 ng/ml 10% CM 50% CM 10 nM 500 nM 10 μM (Fujii et al., 2019)
Basal medium: Advanced DMEM/F12 + Penicinin/Streptomycin (1X) + Glutamine (2 mM) + N2 (1X) + B27 (1X) + N-acetylcysteine (1 mM) + HEPES (10 mM)  
  1. Typically normal organoid and cancer organoid share the same culture medium. aN2 is absent in basal medium. bEGF, Nicotinamide and SB20190 is replaced by 50 ng/ml FGF-2 and 100 ng/ml IGF-1. cAnother TGF-β inhibitor LY2157299 is used instead of A83–01. CM Conditional medium