Fig. 6

(A) In vitro multi-linage differentiation of human adipose tissue derived mesenchymal stem cells (ADSCs): osteogenic differentiation demonstrated by alizarin red stain in the form of dark orange mineral deposits (a), chondrogenic differentiation was stained positive for glucosaminoglycans by Alcain blue stain (b), specific oil red stain indicated adipogenesis induced lipid droplets observed in red colour (c). Scale bars: 200 μm. (B) reverse transcriptase (RT-PCR) determined ADSCs expression of pluripotent markers Nanog, Oct-4 and Sox-2