In vivo generation and regeneration of β cells in zebrafish

Yang, Bingyuan; Covington, Brittney A.; Chen, Wenbiao

doi:10.1186/s13619-020-00052-6

Cell Regeneration

Table 1 Conditions that promote β-cell regeneration

From: In vivo generation and regeneration of β cells in zebrafish

	Inducer	Effects	Developmental stage	Cell source	Related mechanism	Modifer Screen	Gene/target identified	Related pathway	Reference
Manipulation of Insulin signaling	Transgenic expression of a dominant-negative IGF1R	Insulin resistance	Embrynonic, Larval and adult	Progenitors and β cells	Proliferation	None	igf1r	Insulin signalling	(Maddison et al., 2015)
	Knockout of insra/insrb	Insulin resistance	Embryonic and larval	Unknown	N.A.	None	insra/insrb	Insulin signalling	(Yang et al., 2017)
	Transgenic expression of a dominant-negative IRS2	Insulin resistance	Embryonic	Progenitors	Neogenesis	None	irs2	Insulin signalling	(Ye et al., 2016)
	Nutrient	Increased insulin demand	larval	Progenitors	Neogenesis	Candidate drugs	fgf1/rapamycin	FGF, mTORC1	(Maddison & Chen, 2012; Li et al., 2014)
	Nutrient	Increased insulin demand	Larval	Progenitors and β cells	Neogenesis	Candidate drugs	g secretase inhibitor and rapamycin	Notch, mTORC1	(Ninov et al., 2013)
	β-cell ablation	Decreased insulin supply	Larval	Progenitors and β cells	Neogenesis	Chemical library	Adenosine Receptor A2aa (agonist)	Adenosinergic	(Andersson et al., 2012; Schulz et al., 2016)
	β-cell ablation	Decreased insulin supply	Larval	β cells	Proliferation	Chemical library	TBK1/IKKε (inhibitor)	PKA, mTORC1	(Xu et al., 2018)
	β-cell ablation	Decreased insulin supply	Larval	Progenitors	Neogenesis	Mutation	Sox9b	Retinoic acid signaling; Notch signaling	(Manfroid et al., 2012; Huang et al., 2016)
	β-cell ablation	Decreased insulin supply	Larval	Progenitors	Neogenesis	Mutation	Dnmt1	N.A.	(Anderson et al., 2009)
	β-cell ablation	Decreased insulin supply	Larval	Progenitors	Neogenesis	Mutation	fhl1b	N.A.	(Xu et al., 2016)
	β-cell ablation	Decreased insulin supply	Larval	α cells	Trandedifferentiation	Morpholino, antibody	gcg	non-gluconeogenic effects of glucagon	(Ye et al., 2015)
	β-cell ablation	Decreased insulin supply	Larval	α cells	Trandedifferentiation	Mutation	Igfbp1	IGF1 signaling	(Lu et al., 2016)
Chemical genetics	Chemicals	Inhibition or activation of drug targets	Larval	Progenitors	Neogenesis	None	ALDH1A1 (inhibitor), IMPDH (inhibitor)	RA, Nucleotide synthesis	(Rovira et al., 2011; Shen et al., 2013)
	Chemicals	Inhibition or activation of drug targets	Larval	Progenitors and β cells	Proliferation/ Neogenesis	None	NFkb activators, Serotonergic activators	NFkB, Serotonergic, glucorcorticoid analogs	(Wang et al., 2015; Janjuha et al., 2018)
	Chemicals	Inhibition or activation of drug targets	Larval	β cells	Proliferation	None	RXR agonists, serotonin boosters, GR agonists	RA, Serotonergic, glucocorticoid	(Tsuji et al., 2014)
	Notch inhibition	Precocious β-cell differentiation	Larval	Progenitors	Neogenesis	Chemical library	CDK5 (inhibitor)	N.A.	(Liu et al., 2018)
Other	Microbiota	Unknown	Larval	Progenitors	Proliferation	larvae stage	BefA	N.A.	(Hill et al., 2016)
	CDK4	Cell division activation	Larval	β cells	Proliferation	larvae stage	Cyclin-Dependent Kinase 4	N.A.	(Li et al., 2013)
	Genetic Lineage Tracing	Suppression of target gene	embryonic development	Acinar	Trandedifferentiation	None	Ptf1α	N.A.	(Hesselson et al., 2011; Dong et al., 2008)

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