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Cell Regeneration
Fig. 3 | Cell Regeneration

Fig. 3

From: Consistent apparent Young’s modulus of human embryonic stem cells and derived cell types stabilized by substrate stiffness regulation promotes lineage specificity maintenance

Fig. 3

YAP subcellular localization in hESCs and HepaRG cells on different substrate stiffness. YAP translocation in a hESCs and b HepaRG cells during culture on different substrates. Scale bars = 10 μm. c AYM and d YAP translocation (nucleus to cytoplasm) of hESCs on coverslip after being treated with Y27632 and blebbistatin. Scale bar =10 μm. ***P < 0.001. Quantitative analysis of YAP nucleus/cytoplasm ratio in e hESCs and f HepaRG cells. **P < 0.01, ***P < 0.001, ****P < 0.0001. g Schematics of the relationships between AYM and YAP nucleus/cytoplasm ratio in response to substrate stiffness and cell-cell contact

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