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Cell Regeneration
Fig. 4 | Cell Regeneration

Fig. 4

From: Consistent apparent Young’s modulus of human embryonic stem cells and derived cell types stabilized by substrate stiffness regulation promotes lineage specificity maintenance

Fig. 4

Maintenance of pluripotency in hESCs during in vitro culture on substrates with different stiffness. a Brightfield and immunostaining images of OCT-4 for hESCs cultured for 5 days on substrates with different stiffness. Scale bars = 200 μm. b FACS analysis of TRA-1-60 and OCT-4, c OCT-4 and NANOG expression, and d ALP relative activity in hESCs cultured for 5 days on substrates with different stiffness. e OCT-4 and NANOG expression in hESCs after being treated with Y27632 or Verteporfin. f OCT-4 and NANOG expression and g FACS analysis of TRA-1-60 and OCT-4 in up to 3 passages of hESCs on the 3.5 kPa substrate and coverslip. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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