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Fig. 5 | Cell Regeneration

Fig. 5

From: Consistent apparent Young’s modulus of human embryonic stem cells and derived cell types stabilized by substrate stiffness regulation promotes lineage specificity maintenance

Fig. 5

Maintenance of lineage specificity in DE on different substrate stiffness. a Schematic of the differentiation strategy for obtaining hESC-derived DE and hepatoblasts. b AYM measurement of parental DE with the SOX17-GFP reporter using AFM. c Brightfield and immunostaining images of SOX-17 for DE cultured for 5 days on substrates with different stiffness. Scale bars = 200 μm. d Growth curve of DE over 3 passages on the 3.5 kPa substrate and coverslip. SOX17 and FOXA2 expression e in DE cultured for 5 days on substrates with different stiffness and f in 3 passages of DE on the 3.5 kPa substrate and coverslip. FACS analysis of SOX17 and FOXA2 g in DE cultured for 5 days on substrates with different stiffness, and (h) in 3 passages of DE on the 3.5 kPa substrate and coverslip. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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