Fig. 5From: Consistent apparent Young’s modulus of human embryonic stem cells and derived cell types stabilized by substrate stiffness regulation promotes lineage specificity maintenanceMaintenance of lineage specificity in DE on different substrate stiffness. a Schematic of the differentiation strategy for obtaining hESC-derived DE and hepatoblasts. b AYM measurement of parental DE with the SOX17-GFP reporter using AFM. c Brightfield and immunostaining images of SOX-17 for DE cultured for 5 days on substrates with different stiffness. Scale bars = 200 μm. d Growth curve of DE over 3 passages on the 3.5 kPa substrate and coverslip. SOX17 and FOXA2 expression e in DE cultured for 5 days on substrates with different stiffness and f in 3 passages of DE on the 3.5 kPa substrate and coverslip. FACS analysis of SOX17 and FOXA2 g in DE cultured for 5 days on substrates with different stiffness, and (h) in 3 passages of DE on the 3.5 kPa substrate and coverslip. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001Back to article page