Fig. 5

The assessment of other types of cell death potentially involved in erastin-induced ferroptosis in ICCs. The protein expression changes of the protein markers for apoptosis and autophagy were examined after ICCs were challenged by 20 μM erastin for 24 h. Western blot results with the blot images (a) and quantification (b) on the protein expression of BAX, Bcl-2, P62, PARP, cleaved PARP (C-PARP) and LC3 I/II as well as actin were performed and analyzed. (n = 3 per group; *p < 0.05, **p < 0.01, ***p < 0.001. All data are expressed as means ± SEM)