Fig. 3
From: Organoid based personalized medicine: from bench to bedside
Comparison of different culture methods for adult stem cells-derived organoids. In the WENR method, epithelial organoids are derived from tumor biopsies directly in Matrigel with cocktail growth factors, with long-term expansion but no tumor micro environment. In the air-liquid interphase (ALI) method, tumor biopsies are cultured in ALI in the entire tumor microenvironment as a cell suspension of all cell types, including immune cells and other non-epithelial cell types, but with limited expansion