Fig. 7
From: Organoid based personalized medicine: from bench to bedside
Genome editing in organoids by CRISPR-Cas9 technology. Workflow of genetic engineering in organoids using CRISPR-Cas9. Either NHEJ or HR can be exploited for gene knock-out or knock-in, respectively. In most cases, expansion of single organoid clones after the selection procedure is necessary to obtain isogenic organoid populations