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Cell Regeneration
Fig. 1 | Cell Regeneration

Fig. 1

From: Overexpression of HOXA9 upregulates NF-κB signaling to promote human hematopoiesis and alter the hematopoietic differentiation potentials

Fig. 1

Establishment of inducible HOXA9 transgenic hESC lines. a Schematic representation of the piggyBac constructs used to express HOXA9. TRE, tet-on regulation element; CMV mini, cytomegalovirus minimum promoter; T2A, Thosea asigna virus 2A peptide. b After HOXA9/hESCs were induced with DOX for 48 h, the cells were imaged by fluorescence microscopy, allowing observation of co-expressed GFP. c qRT-PCR was used to confirm that inducible expression of HOXA9 was highly stringent and efficient at the transcriptional level. d WB detection was used to confirm HOXA9 overexpression at the protein level, and the pluripotency of HOXA9/hESC using ant-SOX2, OCT4, and NANOG antibodies

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