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Fig. 2 | Cell Regeneration

Fig. 2

From: Overexpression of HOXA9 upregulates NF-κB signaling to promote human hematopoiesis and alter the hematopoietic differentiation potentials

Fig. 2

Overexpression of HOXA9 from D4 promotes hematopoiesis in co-culture with AGM-S3 cells. HOXA9/hESCs were treated with DOX starting from D0, D2, D4, D6, D8, D10, or D12 during co-culture with AGM-S3 cells until D14, and the co-culture cells were subjected to flow cytometry analysis using 7-AAD and the following combinations of antibodies: a KDR (at D4), b CD34/CD43 (at D8), and c GPA/CD71, CD34/CD43, or CD34/CD45 (at D14). Results were compared between non-induced co-cultures and the GFP+ fraction of co-cultures treated with DOX starting on different days. When HOXA9 was induced from the earliest stage, especially D0, CD34 + CD43−, CD34 − CD43+, CD34 + CD43+ populations at D8, and the most hematopoietic populations at D14 were significantly decreased. On the contrary, most above populations at D8 and D14 were dramatically increased by induction of HOXA9 from D4, especially the CD34 − CD45+ population (but GPA + CD71− and GPA + CD71+ populations were decreased in the most case), indicating that HOXA9 strongly promotes hematopoiesis except for the early stage. The experiments were repeated three times, and P < 0.05 was considered significant (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)

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