Fig. 3

Different effects of HOXA9 overexpression on the hematopoietic potential of the mesoderm population at different stages. KDR+ cells were sorted from non-induced HOXA9/hESC co-cultured with AGM-S3 at D1 (a) or D4 (b). About 5 × 10³ sorted cells were re-plated on irradiated AGM-S3 in 24-well plates, treated with or without DOX, and flow cytometry analysis was performed after 10 days using anti-CD34/CD43/CD45 antibodies. The results revealed that the production of CD34+ cells and their subsequent population could be significantly decreased by overexpression of HOXA9 from D0 and continued until D14, whereas their production was significantly increased when overexpression of HOXA9 began on D4 and continued until D14. The experiments were repeated three times, and P < 0.05 was considered significant (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)