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Cell Regeneration
Fig. 4 | Cell Regeneration

Fig. 4

From: Overexpression of HOXA9 upregulates NF-κB signaling to promote human hematopoiesis and alter the hematopoietic differentiation potentials

Fig. 4

The promotion effects of HOXA9 overexpressed on CD34 + CD43+ cells at D8. CD34 + CD43+ cells were sorted from the non-induced HOXA9/hESCs co-cultured with AGM-S3 at D8. About 5 × 103 sorted cells were re-plated in full-lineage hematopoietic differentiation medium (48-well), treated with or without DOX, and flow cytometry analysis was performed after 12 days using 7-AAD and either combination of anti-CD34/CD43, anti-CD34/CD45 or anti-CD43/CD45 antibodies. All these assays revealed a significant increase in the CD34 − CD43+, CD34 − CD45+, and CD43 + CD45+ populations when HOXA9 was overexpressed during further culture. The experiments were repeated three times, and P < 0.05 was considered significant (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)

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