Fig. 5
The positive effects of HOXA9 overexpression on CD34 + CD43+ cells at D14. CD34 + CD43+ cells were sorted from the non-induced HOXA9/hESCs co-cultured with AGM-S3 cells at D14. About 5 × 103 sorted cells were resuspended in 250 μl myeloid or erythroid expansion medium and seeded into each well of a 48-well plate, and then treated with or without DOX. flow cytometry analysis was performed after 8 days using 7-AAD and combination of anti-CD34/CD45 or anti-GPA/CD71 antibodies. All of these assays revealed a significant increase in the CD34 − CD45+ population meanwhile decrease in GPA + CD71+ population when HOXA9 was overexpressed during further culture. The experiments were repeated three times, and P < 0.05 was considered significant (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)