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Cell Regeneration
Fig. 6 | Cell Regeneration

Fig. 6

From: Overexpression of HOXA9 upregulates NF-κB signaling to promote human hematopoiesis and alter the hematopoietic differentiation potentials

Fig. 6

Overexpression of HOXA9 from D4 increases the myelogenic potential of co-cultured cells while decrease their erythrogenic potential. At D14, the non-induced HOXA9/hESC co-culture cells and the GFP+ fractions of D4-induced HOXA9/hESC or GFP/hESC co-culture cells, or CD34 + CD43+ cells sorted from these fractions, were subjected to colony culture assays to detect their hematopoietic potentials. (A) Percentage of each type of colony derived from 2 × 104 co-cultured cells. (B) Typical morphologies of CFU-Mix (a), CFU-E (b), BFU-E (c), and CFU-GM (d) colonies. Scale bar, 100 μm. MGG staining of cells in BFU-E colonies (e). Scale bar, 10 μm. The number of CFU-GM colonies with myelogenic potential increased significantly, but the number of CFU-E colonies with erythrogenic potential decreased, which were not caused by overexpression of GFP. The experiments were repeated three times, and P < 0.05 was considered significant (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)

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