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Fig. 2 | Cell Regeneration

Fig. 2

From: RhoA/Rock activation represents a new mechanism for inactivating Wnt/β-catenin signaling in the aging-associated bone loss

Fig. 2

RhoA interacts genetically with Dkk1 in the limb bud ectoderm of mouse embryos. a Representative immunostaining for p-Rock2 in the E10.5 limb buds with the indicated genotypes. White dot lines separate the apical ectodermal ridge (AER) from the zone of polarizing activity (ZPA). b-d Skeletons and/or limbs of E16.5 embryos with the indicated genotypes. e, f Western analyses in PMCOBs with the indicated genotypes and stimulated with or without rWnt3a at 100 ng/ml for 1 h in the presence or absence of rDkk1 at 100 ng/ml. Phosphorylated proteins were normalized to their total amounts, respectively. g, h H&E and TUNEL staining in E10.5 forelimb bud sections (g) and whole-mount in situ hybridization of E10.5 forelimb buds (h). Dot lines separate the AER from the ZPA. Ventral view for all limb buds, anterior to the lower and posterior to the upper. FL: forelimb, HL: hindlimb

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