Fig. 4

RhoA/Rock activates Jak and GSK3β to destabilize β-catenin in osteoblastic differentiation in response to Wnt3a. a-c Images co-stained Alp with p-Jak2, p-Tyr216-Gsk3β or active β-catenin in proximal tibia (Tb) sections of 2-month-old Col1-Cre, Col1-Cre;dnRhoA^+/−, or Col1-Cre;caRhoA^+/− mice. d The percentages of p-Jak2⁺, p-Tyr216-Gsk3β⁺, or active β-catenin⁺ surface in Alp⁺ surface were quantified. Mean ± SEM, ^**p < 0.01, n = 6, Tukey-Kramer multiple comparisons test. e-j Alizarin Red S staining and quantification or Alp activity assays in C3H10T1/2 cells infected with vector-, Gsk3β(WT)- or Gsk3β(Y216F)-expressing lentiviruses or treated with vehicle or P6 at 50 nM, and further cultured with or without 100 ng/ml of rWnt3a for 21 days or 48 h, respectively. Mean ± SD, *,^¶ p < 0.05, **,⁺⁺p < 0.01, n = 3 ~ 5, Tukey-Kramer multiple comparisons test