Fig. 8

Pharmacological inhibition of Rock by Fasudil antagonizes aging-associated bone loss. 8-month-old male mice were orally administrated with vehicle (Veh.) or Fasudil (Fas.) at 100 mg/kg once daily for 2 months. Tibias were harvested for the following analyses. a, b Representative μCT images and parameters including BV/TV, Tb.Th, Tb.N, and Tb.Sp for the proximal tibias. c, d Representative images of H&E staining and quantification of Ob.N/BS and Ob.S/BS for the proximal tibia sections. e, f Representative images of Trap staining and quantification of Oc.N/BS and Oc.S/BS for the proximal tibia sections. g, h qPCR analyses for Alp, Bsp, Runx2, Lef1, Cyclin D1 (C.D1), and Axin2 mRNA levels in femurs. (i, j) Dynamic bone histomorphometry and quantification for BFR/BS, MAR, and dLS/BS. Mean ± SEM, ^*p < 0.05, ^**p < 0.01, n = 8, Tukey-Kramer multiple comparisons test