Fig. 1
From: Canonical and early lineage-specific stem cell types identified in planarian SirNeoblasts
SirNeoblasts contain the major cell types found in X1 neoblasts. a-b Flow cytometry plots of cells from unirradiated planarians (a) and irradiated planarians (b), stained with SiR-DNA. Red circle indicates the irradiation sensitive cells. Blue circle indicates cells (4 N) for further enrichment. c Flow cytometry plot showing the gating of SirNeoblasts (cells in red circle) by combining the SiR-DNA and Cell Tracker Green. d Images and percentages of piwi-1+ cells in sorted SiR-DNA stained 2 N, 4 N, and SirNeoblsts. e Single cell clustering for SirNeoblasts. f tSNE plot showing cells with high piwi-1+ expression (blue). g Proportions of total cells and high piwi-1+ expression cells in each cluster. h Heatmap of markers for each cluster. i Heatmap correlation analysis for traditional neoblast markers. j-p Violin plots showing the enrichment and expression levels of each neoblast marker