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Fig. 4 | Cell Regeneration

Fig. 4

From: Canonical and early lineage-specific stem cell types identified in planarian SirNeoblasts

Fig. 4

Pluripotent stem cells are enriched in CS0 and CS1 subclusters. a KEGG enrichment for genes in CS1 and CS0. b Phylogenic tree of gcm in the GCM transcription factor family. Dm, Drosophila melanogaster. Nv, Nasonia vitripennis. Bg, Biomphalaria glabrata. Lg, Lottia gigantea. Ct, Capitella teleta. Hs, Homo sapiens. Mm, Mus musculus. c tSNE plot showing SMED30023953 (gcm) + cells in blue. d tSNE plot showing gcm + tgs-1+ cells in blue. e FISH showing co-localization of gcm with piwi-1. White box indicates inset with high magnification in column second from left. piwi-1+ (magenta); gcm (green); phosphorylated histone 3 (H3P) (white); nuclei (blue) indicate antibodies/channels. Arrows indicate a piwi-1 + gcm + H3P+ cell. Scale bars indicate 200 μm. f FISH showing co-localization of gcm with tgs-1. White box indicates inset with high magnification in column second from left. gcm + (magenta); tgs-1 (green); H3P (white); nuclei (blue) indicate antibodies/channels. Arrows indicate a tgs-1 + gcm + H3P+ cell. Scale bars indicate 200 μm. g Unirradiated egfp (RNAi) and gcm (RNAi) planarians stained for tgs-1 FISH (green), anti-H3P antibody (white) and DAPI (blue). h egfp (RNAi) and gcm (RNAi) planarians at 7 dpi and 14 dpi stained for piwi-1 FISH. i Quantification of piwi-1+ cells/mm2 shows impaired recovery of neoblasts in gcm (RNAi) versus egfp (RNAi) planarians. *, p < 0.05

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