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Cell Regeneration
Fig. 2 | Cell Regeneration

Fig. 2

From: Forkhead box family transcription factors as versatile regulators for cellular reprogramming to pluripotency

Fig. 2

KS FoxD3/ FoxD4/ FoxG1 derived iPSCs resemble mouse ESCs. a Representative images of KSFoxs-iPSCs at day 8 after induction. These iPSCs could be passed normally. Scale bar, 250 μm. b Genome PCR showed that these KSFox-iPSCs were free of Oct4 transgene contamination. c RT-PCR analysis of the expression levels of pluripotent marker genes in KSFoxD3/ FoxD4/ FoxG1 derived iPSCs; n = 3. d KSFoxD3 iPSCs colonies expressed pluripotency markers Oct4, Nanog and SSEA-1. DAPI stating was used as control. Scale bar, 100 μm. e The KSFoxD3-iPSCs colonies had normal karyotype. f Three germ layer reprentative tissues presented in teratomas derived from KSFoxD3-iPSCs. g Chimeric mice and germline transmission generated from KSFoxD3-iPSCs. h Retroviral integrations of KSFoxD3-iPSCs analyzed by PCR with genomic DNA

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