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Fig. 3 | Cell Regeneration

Fig. 3

From: Forkhead box family transcription factors as versatile regulators for cellular reprogramming to pluripotency

Fig. 3

FoxO6 hinders somatic cell reprogramming. a Representative images showed the reprogramming process that MEFs were transduced with OKS + Ctrl and OKS + FoxO6. scale bars, 250 μm. b The number of Oct4-GFP colonies was counted at day 7 with or without FoxO6 in OKS mediated reprogramming system; n = 3. Representative fields of GFP+ colonies taken by fluorescence microscope in situ at day 7. Scale bar, 250 μm. c The proportions of Oct4-GFP positive colonies reflected reprogramming efficiency according to flow cytometry analysis. d Growth curves of OKS + Ctrl and OKS + FoxO6 induced conditions; n = 3. e RT-qPCR analysis showed the relative expression of representative genes related to pluripotency and MET process in reprogramming; n = 3. f Schematic diagrams illustrating the various truncated types of FoxO6. Forkhead domain, KIX-binding domain, and transactivation domain were shown as red rectangle, yellow rectangle and blue rectangle respectively. g Effects of the various truncated types of FoxO6 in OKS-mediated reprogramming; n = 3

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