Fig. 3

Mc4r-GFP expression in the skeleton of embryonic mouse limb. a-h Representative images of Mc4r-GFP and Sox9 expression detected by immunofluorescence staining, on sections of E11.5 hindlimb (a,b), E13 forelimb (c,d), E16 forelimb (e,f), and E18.5 hindlimb digit 3 (g,h). (b,d,f,h) are higher magnification of outlined areas in (a,c,e,g). i-k Expression of Mc4r-GFP and Col 2 (i), Col X (j) and OPN (k) on section of E18.5 hindlimb digit 3. Images are shown with distal to the left. Scale bars: 200 μm in (a,c,e,g); 50 μm in (b,d,f,h, i-k)