Fig. 2
From: Coordination of EZH2 and SOX2 specifies human neural fate decision
EZH2−/− hESCs aberrantly express meso/endoderm genes during neural induction. a qRT-PCR analysis of the expression of the pluripotent genes OCT4/NANOG, the NPC genes SOX2/SOX1/PAX6/FOXG1, and meso-endoderm genes CDH1/ZIC1/PAX3/ERBB3/TFAP2A/ FOXD3/BMP4/KDR/CXCL12/FOXH1/KRT8/KRT18/KRT19/VEGFA/CLDN6 at day 0, day 8 and day 16 during neural differentiation. Wild-type H1 hESCs served as controls. The data represent the mean ± SD (standard deviation) from three independent replicates (n = 3). Significance was determined using unpaired two-tailed Student’s t-tests. **, P < 0.01. *, P < 0.05. b Left panel, heatmap of up- or downregulated genes in EZH2-mutant and wild-type cells at day 8 of neural differentiation (EZH2−/−-D8 and WT-D8, respectively). Right panel, GO analysis for up- or downregulated genes in EZH2-mutant cells at day 8 of neural differentiation. c. Heatmap analysis for genes mentioned in Fig. 2a