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Fig. 3 | Cell Regeneration

Fig. 3

From: Establishment of porcine and monkey colonic organoids for drug toxicity study

Fig. 3

Mature lineages of monkey colonic organoids are induced in differentiation medium. A, B Representative images (A) and quantitation of organoid size (B) of monkey colonic organoids cultivated in expansion or differentiation medium for 7 days. White box depicts higher magnification below. C Expression of cell marker genes in proliferating (EM), differentiated (DM) monkey colonic organoids (passage 15) and tissue. D, E Fabp1 staining (D), Muc2 staining (E) of monkey colonic organoids cultured with expansion or differentiation medium for 7 days. Scale bars, 100 μm. *p < 0.05, ** p < 0.01, ***p < 0.001 analyzed by Student’s t-test. Data are shown as mean ± SD (n = 3 independent experiments)

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