Fig. 1

Cloning and identification of murine and human CVSCs. a Schematic shows the selective culture of putative CVSCs from the mouse cervix and representative phase-contrast microscopic image presenting primary cell colony. Scale bar, 100 μm. b Colony forming efficacy of CVSCs of different culture passages. Data representative of n = 3 biological replicates. p, passage. c Putative mouse CVSC colonies stained with proliferative marker KI67, cervical epithelial stem cell markers KRT5, KRT17, and P63, cervical squamous cell marker KRT10, and cervical columnar cell marker MUC1. Data representative of n = 10 biological replicates. Scale bar, 100 μm. d Relative expression levels of genes frequently upregulated in epithelial stem cells and genes considered as differentiated cervical epithelial markers. e Representative phase-contrast microscopic image of putative human CVSC colonies. Scale bar, 100 μm. f Colony forming efficacy and representative karyotype of human CVSCs. Cells for karyotyping were harvested at passage 15. Data representative of n = 3 biological replicates. p, passage. g Putative human CVSC colonies stained with human-specific marker human nuclear antigen (HUNU), and adult epithelial stem cell markers KRT5, KRT17, and P63. Data representative of n = 3 biological replicates. Scale bar, 100 μm