Fig. 3
From: Single-cell analysis defines the lineage plasticity of stem cells in cervix epithelium
Cervix epithelium regeneration in the TCA injury model. a Experimental scheme. 6–8-week-old female mice were treated with 2.5% TCA via direct injections into the cervix, and the cervix samples were collected at three time points post-injection (Day3, Day10, and Day20) for histological analysis. Data representative of n = 5 biological replicates. b Expression of P63 and KRT10 along the endo-cervical epithelium at indicated days after TCA injury. Enlarged inset outlined by yellow: P63 + KRT10+ cells showing initiation of squamous metaplastic formation after injury. Sham cervix served as a negative control. Data representative of n = 5 biological replicates per time point. Scale bar, main panel, 100 μm; enlarged inset,10 μm. c P63+ and KRT10+ cell frequency in the injured endo-cervix epithelium. Data representative of n = 5 biological replicates per time point. d KRT10- and KRT10+ cell fraction in P63+ cells at designated post-injury time points was plotted as mean ± SD. Data representative of n = 5 biological replicates per time point. e Lineage tracing experiments in Krt5 CreERT2-Gt(ROSA)26Sortm4(ACTB-tdTomato-EGFP) mice treated with low-dose tamoxifen prior to TCA injury reveals that squamous metaplasia arising after the injury is positive for Krt5-GFP lineage marker on Day3. Data representative of n = 5 biological replicates. Scale bar, 100 μm