Fig. 2

Characterization of brain organoids derived from hiPSC on the micropillar chip. A Bright-field images of developing brain organoids at different days. Scale bar: 200 μm. B, C Immunohistochemical analysis were conducted to detect the NESTIN⁺/SOX2⁺ neural progenitor cells, TUJ1⁺ differentiated neurons, PAX6⁺ forebrain, BRN2⁺ and TBR1⁺ cortical layer, vGLUT1⁺ excitatory neurons and GABA⁺ inhibitory neurons in day-38 and day-70 brain organoids. Scale bars indicated in the image