Fig. 6

Linc-RAM regulates the enzymatic activity of PYGM. A, B Enzymatic activity of PYGM in C2C12 cells transfected with plasmids expressing Linc-RAM (OE) or empty vector as control (NC) and cultured in growth medium (A) or differentiation medium for 24 h (B). C, D Enzymatic activity of PYGM in primary myoblasts isolated from skeletal muscle of Linc-RAM-knockout mice (KO) or wild-type (NC) littermates and cultured in growth medium (C) or differentiation medium for 24 h (D). E Enzymatic activity of PYGM examined in skeletal muscle (gastrocnemius) from Linc-RAM-knockout mice (KO) or wild-type (NC) littermates. n = 3 per genotype. Values presented represent the means ± SE obtained from three independent experiments (A-D). The statistical significance of the difference between two means was calculated with the Student’s t-test. *p < 0.05