Fig. 3
From: Chchd10 is dispensable for myogenesis but critical for adipose browning
Chchd10 knockout does not affect muscle development and satellite cell mediated muscle growth and regeneration; but reduces grip strength and exercise performance in mice. A Chchd10 knockout (KO) strategy using CRISPR-CAS9 targeting to delete Exon 3 which truncated the functional CHCH domain. MTS: mitochondrial targeting sequence; ND: non-domain sequence. B Western blot confirming the lack of CHCHD10 protein in the KO TA muscles. N = 3. C H&E cross-sectional staining of representative regenerated areas in uninjured (upper) and injured TA muscles at 5 day post injured (dpi; bottom) TA muscle, scale bar: 100 μm. D-E Double immunofluorescence staining of PAX7 and α-LAMININ (D, to mark satellite cells), or MYOG and DYSTROPHIN (E, to mark newly differentiated myoblasts) in TA muscle cross-sections at 5 dpi. Scale bar: 100 μm. F-G Grip strength (F) and treadmill running distance (G) for WT and Chchd10KO mice. * p < 0.05. N = 6 for F and n = 3 for G