Skip to main content
Fig. 2 | Cell Regeneration

Fig. 2

From: miR-378-mediated glycolytic metabolism enriches the Pax7Hi subpopulation of satellite cells

Fig. 2

Delayed muscle regeneration in miR-378 transgenic mice. A Frozen sections from the TA muscle of miR-378 TG and WT mice damaged for 7 days were immunostained for laminin (red) and DAPI (green) (n = 6 mice per group). B The cross-sectional area of regenerated myofibers with centralized nuclei was calculated based on the immunostaining in panel (A). y axis represents percentage of myofibers with centralized nuclei. C Representative views of MyoD (green) and BrdU (red) staining on sections of TA muscle 1.5 days post-CTX injury. DAPI (blue) served to visualize nuclei. D Number of MyoD and BrdU double positive cells calculated in TA muscle sections described in panel (C). E Relative mRNA level of MyoD in TA muscle 1.5 days post injury from the miR-378 TG mice and WT littermates, determined by RT-qPCR. F-G Relative mRNA levels of Pax7 (F) and Myh3 (G) in TA muscle 7 days post injury from the miR-378 TG mice and WT littermates, determined by RT-qPCR. H Representative images of immunostained Pax7 on cryosections of CTX-damaged TA muscles from the miR-378 TG mice and WT littermates 30 days post injury. Scale bars, 50 μm. I Number of Pax7 positive cells calculated on TA sections described in panel (H), were normalized to WT controls which were set up to 1. J Normalized Pax7 positive cell number in TA muscle at 30 days after the second round of CTX-induced injury. *P < 0.05, **P < 0.01. Two-tailed Student’s t-test was used for all statistical tests

Back to article page