Fig. 3

Enriched Pax7^Hi subpopulation of satellite cells in miR-378 TG mice. A Pax7 protein in TA muscle of miR-378 TG and WT mice was detected by western blot. GAPDH was used as a loading control. B A representative view of Pax7 (red) staining on sections of TA muscle from the miR-378 TG mice and WT littermates. DAPI (blue) served to visualize nuclei. Scale bars, 50 μm. C Number of Pax7-positive cells in the immunostained sections described in (B). D The percentage of the Pax7^Hi subpopulation was calculated by FACS. E Expression of stemness-, differentiation- and mitochondria-related markers determined using real-time RT–PCR in sorted satellite cells from miR-378 TG and WT mice. F Satellite cells from the Pax7-nGFP;miR-378 TG mice and Pax7-nGFP controls were sorted using FACS, cultured for 24 h in growth medium and immunostained for MyoD (red). DAPI (blue) served to visualize nuclei. G Numbers of MyoD positive cells calculated in panel (F). Values are the means ± s.e.m. of triplicate experiments. *P < 0.05, **P < 0.01. Two-tailed Student’s t-test was used for all statistical tests