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Fig. 2 | Cell Regeneration

Fig. 2

From: Myogenesis controlled by a long non-coding RNA 1700113A16RIK and post-transcriptional regulation

Fig. 2

1700113A16Rik was localized in both cytoplasm and nucleus of muscle cells and enriched in the cytoplasm of muscle cells. A RT-qPCR was performed with RNA extracted from the cytoplasm and nucleus of MuSCs before and after differentiation to detect the distribution of 1700113A16Rik. 1700113A16Rik was upregulated during MuSC differentiation and enriched in the cytoplasm of MuSCs. Error bars indicated standard deviation and were based on 3 independent experiments. B Immunofluorescent staining of MyoD and MyHC. C2C12 myoblasts and the differentiated C2C12 cells were subjected for immunofluorescent staining with MyoD and MyHC antibody. Green indicated MyoD; Red indicated MyHC; Blue indicated DAPI; Merge indicated the merge of red, green and blue images. (Scale Bars, 200 μm). C RNA in situ hybridization was performed to detect the distribution of 1700113A16Rik in C2C12 cells before and after differentiation (Scale Bars, 50 μm)

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