Fig. 5

1700113A16Rik promoted MuSCs differentiation by targeting MEF2D. A The locations of 1700113A16Rik and MEF2D in the genome. The 3’UTR of Mef2d complementary paired with 1700113A16RIK. B The relative RNA level of 1700113A16Rik in the differentiated MuSCs. MuSCs were transfected by two pieces of siRNA against 1700113A16Rik (si1700113A16Rik-1 and si1700113A16Rik-2) or scramble RNA (siNC) and then differentiated for 3 days. RT-qPCR assays were performed with RNA extracted from the differentiated MuSCs in the control group and the 1700113A16Rik knockdown group. Error bars indicated standard deviation and were based on 3 independent experiments. * indicated p<0.05, ** indicated p<0.01. C The relative RNA level of Mef2d in the differentiated MuSCs described in B. RT-qPCR assays were performed with RNA extracted from the differentiated MuSCs in the control group and the 1700113A16Rik knockdown group. Error bars indicated standard deviation and were based on 3 independent experiments. NS indicated no significant changes. D The protein level of MEF2D in the differentiated MuSCs described in B. Western blot was performed from the differentiated MuSCs in the control group and the 1700113A16Rik knockdown group. E The relative RNA level of 1700113A16Rik in the differentiated MuSCs. MuSCs were infected by the control adenovirus and adenovirus encoding 1700113A16Rik and then differentiated for 3 days. RT-qPCR assays were performed with RNA extracted from the differentiated MuSCs in the control group and the 1700113A16Rikoverexpressing group. Error bars indicated standard deviation and were based on 3 independent experiments. *** indicated p<0.001. F The relative RNA level of Mef2d in the differentiated MuSCs described in E. RT-qPCR assays were performed with RNA extracted from the differentiated MuSCs in the control group and the 1700113A16Rik-overexpressing group. Error bars indicated standard deviation and were based on 3 independent experiments. NS indicated no significant changes. G The protein level of MEF2D in the differentiated MuSCs described in E. Western blot was performed from the differentiated MuSCs in the control group and the 1700113A16Rik-overexpressing group. The numbers below each panel indicated the relative signal intensity. H The luciferase activity of reporter gene carrying 1700113A16Rik target sequence from Mef2d at the 3’UTR region. Firefly luciferase activity regulated 1700113A16Rik target sequence at the 3’UTR. Renilla luciferase activity driven by CMV promoter served as an internal control. Error bars indicated standard deviation and were based on 5 independent experiments. * indicated p<0.05. NS indicated no significant changes