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Fig. 6 | Cell Regeneration

Fig. 6

From: Cross-species single-cell transcriptomic analysis reveals divergence of cell composition and functions in mammalian ileum epithelium

Fig. 6

Divergence of drug absorption in ileum. A, Expression heatmap of signature genes in enterocytes and functional enrichments among five species. B, Pearson correlation of scRNA-seq expression (log1p (average expression)) for drug metabolism and transporter genes. Species from top to bottom: macaque versus human, pig versus human, rat versus human and mouse versus human. C, Violin plots showing the expression of drug metabolism and drug transporter genes across human, macaque, pig, rat and mouse. ND, not detected. D, Immunofluorescence was performed to confirm the CES1 and CES2 expression in macaque, pig and human ileum. Scale bars, 100 μm. E, The expression of CYP3A family members in differentiated mouse, pig and human ileum organoids relative to β-actin, and its activity was examined by P450-Glo CYP3A4 assay kit. F SLC22A1 expression relative to β-actin and metformin uptake in mouse, pig and human ileum organoids. Data are displayed as the mean ± SD (n = 3 independent experiments). ns (no significance); *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t-test (E and F)

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