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Fig. 1 | Cell Regeneration

Fig. 1

From: DDB1 maintains intestinal homeostasis by preventing cell cycle arrest

Fig. 1

DDB1 maintains intestinal homeostasis by preventing cell cycle arrest. A Immunofluorescence (IF) staining to detect DDB1 expression in Ctrl (DDB1fl/fl) and KO (DDB1fl/fl;Villin-CreERT2) small intestine at day 4 after the first TAM injection. The day with first TAM injection is regarded as day 0. Scale bar, 100 μm. B Representative images of Ctrl and KO intestine at day 8. Scale bar, 5 cm. The length of intestine is quantified in Fig. S1E. C Hematoxylin and eosin (H&E) staining of proximal and distal small intestine from Ctrl and KO mice at day 4, day 6 and day 8. Scale bar, 100 μm. The viable crypt number is quantified in Fig. S2A. D IF staining of Ki67 and endogenous Lgr5-EGFP in Ctrl and KO small intestine at day 4. Scale bar, 100 μm. The number of Ki67+ cells and Lgr5+ crypts are shown in Fig. S3B and S4A respectively. E Gene ontology (GO) analysis of upregulated genes in KO small intestinal crypts at day 2, obtained from RNA-seq (n = 2). F Immunoblotting of small intestinal crypts and villi from Ctrl and KO mice at day 2. G Cell cycle analysis of Lgr5+ ISCs from Ctrl and KO mice at day 2. Data are presented as mean ± SD (n = 3). Student’s t-test, *P < 0.05. H UC2288 treatment of Lgr5-EGFP labeled small intestinal organoids for 4 days with or without DDB1 deletion. DDB1 deletion in organoids is induced by 4-OHT. Scale bar, 100 μm. The budding number and Lgr5+ cell ratio of organoids are shown in Fig. S6B and S6C, respectively

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