Fig. 1

Transcriptomic correlation analysis reveals a functional link of Rif1 to RNF2 and Pcgf6. A Known regulators controlling the pluripotency-to-totipotency transition of mESCs. B STRING visualization of the protein-protein interaction network of the previously reported 21 repressors of totipotency. C Principal component analysis (PCA) of gene expressions of the mESCs with depletion of the indicated genes. D PCA based on the expressions of repetitive elements. E Correlation matrix showing the unbiased and pairwise comparisons of the global changes in gene expression upon depletion of the indicated genes in mESCs. F Correlation matrix generated with the transcriptional changes of repetitive elements upon depletion of the indicated genes in mESCs. Color bars represent Pearson correlation coefficient. G Venn diagram illustrating the differentially expressed genes (adjusted p-value < 0.05 and |Log₂ Fold Changes (FC)| > 1) in the mESCs depleted of Rif1, Pcgf6, or RNF2. H Venn diagram of the differentially expressed repetitive elements (adjusted p-value < 0.05 and |Log₂ FC| > 1)