Fig. 3From: Rif1 interacts with non-canonical polycomb repressive complex PRC1.6 to regulate mouse embryonic stem cells fate potentialSpecific interaction between Rif1 and Pcgf6. A Schematic illustrating the LacO-LacI system used for detection of protein-protein interactions. B The LacO-LacI induced ectopic colocalization experiments reveal a specific interaction between Rif1 and Pcgf6. GFP-Rif1 is shown in green, LacI-DsRed fused with the indicated bait proteins are shown in red. DNA is stained with DAPI (blue). Bar: 10 μm. C The method to calculate the relative enrichment of GFP-Rif1. D The relative enrichment of GFP-Rif1 in the indicated experimental groups. ns: not significant, **** p < 0.0001 by t-test, Error bars represent SD, n = 12–45 cells per group. E Coimmunoprecipitation of HA-Rif1 with different Flag-tagged Pcgf proteins ectopically expressed in HEK293T cellsBack to article page