Fig. 7
Rif1 and Pcgf6 coregulates a group of MERVL. A Top: schematic of the 2C::tdTomato reporter. Bottom: fluorescent images showing the fraction of tdTomato-positive cells in control or mESCs treated with the indicated shRNA. Control, non-transfected; shNT, non-target shRNA transfected. Bar: 50 μm. B RT-qPCR analysis of the indicated transcripts after the transfection of the corresponding shRNAs in the 2C::tdTomato reporter cell line. **** p < 0.0001 by t-test, Error bars represent SD, n = 3. C The percentages of tdTomato-positive cells in the indicated experimental groups. ns: not significant, **** p < 0.0001 by t-test, Error bars represent SD, n = 16–26 fields per group. In each field, the number of total cells is approximately 150–350. D Fluorescent images showing the fraction of tdTomato-positive cells in control or mESCs treated with the indicated shRNA. shNT, non-target shRNA transfected. Bar: 25 μm. E The percentages of tdTomato-positive cells in the indicated experimental groups. ns: not significant, **** p < 0.0001 by t-test, Error bars represent SD, n = 20–26 fields per group. In each field, the number of total cells is approximately 150–350. F Heatmap of RNA-seq data showing the expression levels of the Rif1-bound MERVL loci that are derepressed upon Rif1 depletion (n = 66) and their corresponding expression levels in mESCs after Pcgf6 knockdown. A fraction of these MERVL loci (n = 48) becomes activated in Pcgf6 KD mESCs. The color bar denotes the Log2(CPM + 1). G Schematic model of Rif1 as a new auxiliary component of the PRC1.6 complex