Fig. 1
From: Cilia regeneration requires an RNA splicing factor from the ciliary base
The localization of PRP-8 in the ciliated neurons of C. elegans. A A cartoon model of the nematode C. elegans. Amphid cilia, amphid nuclei, and phasmids are indicated in yellow boxes. B Schematic model of the auxin-induced degradation system in the absence of auxin (or IAA, indole acetic acid). Spliceosomal component PRP-8, labeled with degron sequence, can not be targeted to degradation in the phasmids of C. elegans. GFP11 can combine with GFP1–10 and therefore mark PRP-8 with green fluorescence, specifically in sensory neurons. No GFP signal can be detected in other tissues, as GFP1–10 is expressed under the control of the ciliated neuron-specific Pche-3 promotor. C PRP-8 localizes at the ciliary base. The full-length cilia are visualized by IFT particle marker DYF-11::wrmScarlet. m.s., middle segment; d.s., distal segment. D Localization of PRP-8 (labeled with GFP, green) or ciliary marker DYF-11 (labeled with wrmScarlet, magenta) in the amphids (upper and middle) or phasmids (lower). In the absence of auxin, PRP-8 in sensory neurons localizes both in the nuclei (E) and at the ciliary bases (F). Scale bar, 10 μm. E Enlarged phasmid nuclei indicated in (D). F Enlarged phasmid cilia indicated in (D). Ciliary bases are indicated by white arrows