Fig. 3
From: Cilia regeneration requires an RNA splicing factor from the ciliary base
Restoration of PRP-8 and the regeneration of cilia. A Schematic model of the restoration of PRP-8. TIR1 no longer binds degron-tagged PRP-8 when auxin is removed. Thus newly synthesized PRP-8 is not degraded by the proteasome. B Localization of PRP-8 in the phasmids before or after removal of auxin for 24 hours. The worm was treated with 4 mM auxin on NGM plates for 24 hours before removal of auxin. Phasmid nuclei are indicated in gray boxes (C-D). Phasmid cilia are indicated in gray arrows (E-F). Scale bar, 2 μm. C Enlarged phasmid nuclei before recovery indicated in (B). D Enlarged phasmid nuclei after recovery indicated in (B). E Enlarged phasmid cilia before recovery indicated in (B). F Enlarged phasmid cilia after recovery indicated in (B). Ciliary bases are indicated by white arrowheads. Scale bar, 2 μm. G Representative images of regenerated cilia after recovery for 24 hours. The same cilia from the same worm are compared. Ciliary tips are indicated by white arrowheads. Scale bar, 2 μm. H Cilia length before or after removal of auxin for 24 hours. 10 regenerated cilia are counted. I Ciliary basal PRP-8 intensities before or after removal of auxin for 24 hours. 10 regenerated cilia are counted. The three cilia with decreased ciliary PRP-8 intensities after recovery are indicated in magenta in (H) and (I). J Percentage of abnormal cilia before or after removal of auxin for 48 hours. For each worm, two cilia from different phasmids are counted. n indicates the number of cilia. ***P < 0.001 via Fisher’s exact test. K Quantification of cilia length before or after removal of auxin for 48 hours. Normal or abnormal cilia correspond to those in (J). n indicates the number of cilia. L Summary of IFT velocities. Numbers of IFT particles are shown in the brackets. IAA (−) group, before auxin treatment; IAA (+) group, after auxin treatment for 24 hours; Recovery group, after recovery for 24 hours; Antero., anterograde; m.s., middle segment; d.s., distal segment. **P < 0.01 and ****P < 0.0001 and ns denotes P > 0.05 via unpaired t-test. M Kymographs of cilia before or after removal of auxin for 24 hours. Anterograde IFT particles are marked with yellow lines. Retrograde IFT particles are marked with blue lines. t.z., transition zone. Scale bar (horizontal), 2 μm; Scale bar (vertical), 2 s