Fig. 4
From: Cilia regeneration requires an RNA splicing factor from the ciliary base
Cilia regeneration and working model. A Histograms of IFT velocity. IAA (−), IFT particles in cilia before auxin treatment; IAA (+), IFT particles in defective cilia after 4 mM auxin treatment for 24 hours; Recovery, IFT particles in regenerated cilia which are defective after auxin treatment but regenerated when recovery. m.s., middle segment; d.s., distal segment. B A working model illustrating the dependency of ciliogenesis and cilia regeneration on ciliary basal PRP-8 rather than nuclear PRP-8. After 8-hour auxin treatment, PRP-8 in the phasmid nuclei instead of ciliary bases was degraded, and cilia remained intact; After 24-hour auxin treatment, both PRP-8 in the nuclei and at the ciliary bases were degraded, thus ciliary defects could be seen; Further, after 48-hour auxin-free recovery, cilia regenerated along with restoration of ciliary PRP-8 rather than nuclear PRP-8